Life Science Buffers

Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.

Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.

Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.

The choice of buffer for a particular biological reaction or site depends on several factors:

Temperature
Desired or target pH
Buffer toxicity (to the system)
Buffer interactions with other system components

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316 – 330 of 3,516 results

316

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Thermo Scientific Chemicals MES, 99+%, <1% water

CAS: 4432-31-9 Molecular Formula: C6H13NO4S Molecular Weight (g/mol): 195.233 MDL Number: MFCD00006181 InChI Key: SXGZJKUKBWWHRA-UHFFFAOYSA-N Synonym: 2-(N-Morpholino)ethanesulfonic acid PubChem CID: 78165 ChEBI: CHEBI:39005 IUPAC Name: 2-morpholin-4-ylethanesulfonic acid SMILES: C1COCCN1CCS(=O)(=O)O

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Specifications

PubChem CID	78165
CAS	4432-31-9
Molecular Weight (g/mol)	195.233
ChEBI	CHEBI:39005
MDL Number	MFCD00006181
SMILES	C1COCCN1CCS(=O)(=O)O
Synonym	2-(N-Morpholino)ethanesulfonic acid
IUPAC Name	2-morpholin-4-ylethanesulfonic acid
InChI Key	SXGZJKUKBWWHRA-UHFFFAOYSA-N
Molecular Formula	C6H13NO4S

317

Thermo Scientific Chemicals CAPS, 0.5M buffer soln., pH 10.5

CAS: 1135-40-6 Molecular Formula: C9H19NO3S Molecular Weight (g/mol): 221.32 MDL Number: MFCD00003837 InChI Key: PJWWRFATQTVXHA-UHFFFAOYSA-N IUPAC Name: 3-(cyclohexylamino)propane-1-sulfonic acid SMILES: OS(=O)(=O)CCCNC1CCCCC1

Pricing & Availability
Specifications

CAS	1135-40-6
Molecular Weight (g/mol)	221.32
MDL Number	MFCD00003837
SMILES	OS(=O)(=O)CCCNC1CCCCC1
IUPAC Name	3-(cyclohexylamino)propane-1-sulfonic acid
InChI Key	PJWWRFATQTVXHA-UHFFFAOYSA-N
Molecular Formula	C9H19NO3S

318

Thermo Scientific Chemicals TRIS-buffered saline (TBS, 10X) pH 8.0

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

Pricing & Availability
Specifications

PubChem CID	6503
CAS	77-86-1
Molecular Weight (g/mol)	121.136
ChEBI	CHEBI:9754
MDL Number	MFCD00004679
SMILES	C(C(CO)(CO)N)O
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

319

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PIPES, Monosodium Salt, Biological Buffer, High Purity, 98.5%, Spectrum™ Chemical

CAS: 10010-67-0 Molecular Formula: C8H17N2NaO6S2 Molecular Weight (g/mol): 324.34 MDL Number: MFCD00065472 InChI Key: OGGAIRCLBMGXCZ-UHFFFAOYSA-M IUPAC Name: sodium 2-[4-(2-sulfoethyl)piperazin-1-yl]ethane-1-sulfonate SMILES: [Na+].OS(=O)(=O)CCN1CCN(CCS([O-])(=O)=O)CC1

Pricing & Availability
Specifications

CAS	10010-67-0
Molecular Weight (g/mol)	324.34
MDL Number	MFCD00065472
SMILES	[Na+].OS(=O)(=O)CCN1CCN(CCS([O-])(=O)=O)CC1
IUPAC Name	sodium 2-[4-(2-sulfoethyl)piperazin-1-yl]ethane-1-sulfonate
InChI Key	OGGAIRCLBMGXCZ-UHFFFAOYSA-M
Molecular Formula	C8H17N2NaO6S2

320

Gibco™ PBS, pH 7.4

Gibco™ Balanced Salt Solutions are sterile-filtered, packaged in bioprocess containers, and ready to order.

Pricing & Availability
Specifications

Color	Clear, colorless solution
Physical Form	Liquid
pH	7.3 to 7.5
Manufacturing Quality	cGMP
Chemical Name or Material	Phosphate Buffered Saline (PBS)
Osmolality	280 to 315 mOsm/kg
Sterility	Sterile-filtered

321

MES lysis buffer with Triton™ X-100, Thermo Scientific™

Pricing & Availability
Specifications

Health Hazard 3	P264b-P280i-P305+P351+P338
Color	Colorless
Physical Form	Liquid
Health Hazard 1	H319
Chemical Name or Material	MES lysis buffer with Triton™ X-100
TSCA	No
Recommended Storage	Keep cold

322

Thermo Scientific Chemicals TRIS-glycine-native running buffer (10X), pH 8.5

Pricing & Availability
Specifications

Packaging	Plastic bottle
Physical Form	Liquid
Chemical Name or Material	TRIS-glycine-native running buffer (10X)
TSCA	Yes
Recommended Storage	Ambient temperatures

323

BES, 99+%, MP Biomedicals™

CAS: 10191-18-1 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.25 MDL Number: MFCD00007533 InChI Key: AJTVSSFTXWNIRG-UHFFFAOYSA-N Synonym: n,n-bis 2-hydroxyethyl-2-aminoethanesulfonic acid,bes,ethanesulfonic acid, 2-bis 2-hydroxyethyl amino,2-bis 2-hydroxyethyl amino ethanesulfonic acid,n,n-bis 2-hydroxyethyl taurine,n,n-bis hydroxyethyl-2-aminoethanesulfonic acid,bes buffering agent,taurine, n,n-bis 2-hydroxyethyl,2-bis 2-hydroxyethyl amino-ethanesulfonicacid PubChem CID: 73243 ChEBI: CHEBI:39045 IUPAC Name: 2-[bis(2-hydroxyethyl)amino]ethane-1-sulfonic acid SMILES: OCCN(CCO)CCS(O)(=O)=O

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Specifications

PubChem CID	73243
CAS	10191-18-1
Molecular Weight (g/mol)	213.25
ChEBI	CHEBI:39045
MDL Number	MFCD00007533
SMILES	OCCN(CCO)CCS(O)(=O)=O
Synonym	n,n-bis 2-hydroxyethyl-2-aminoethanesulfonic acid,bes,ethanesulfonic acid, 2-bis 2-hydroxyethyl amino,2-bis 2-hydroxyethyl amino ethanesulfonic acid,n,n-bis 2-hydroxyethyl taurine,n,n-bis hydroxyethyl-2-aminoethanesulfonic acid,bes buffering agent,taurine, n,n-bis 2-hydroxyethyl,2-bis 2-hydroxyethyl amino-ethanesulfonicacid
IUPAC Name	2-[bis(2-hydroxyethyl)amino]ethane-1-sulfonic acid
InChI Key	AJTVSSFTXWNIRG-UHFFFAOYSA-N
Molecular Formula	C6H15NO5S

324

Transformation buffer-2, pH 7.5, Thermo Scientific™

For transformation of competent cells

Pricing & Availability
Specifications

Physical Form	Liquid
pH	7.5
Recommended Storage	Keep cold

325

Thermo Scientific Chemicals Hanks' Balanced Salt Solution (10X), with calcium, with magnesium, without phenol red

Pricing & Availability
Specifications

Color	Colorless
Physical Form	Liquid
Recommended Storage	Ambient temperatures

326

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Bis-Tris Hydrochloride, Biological Buffer, 99%, Spectrum™ Chemical

CAS: 124763-51-5 Molecular Formula: C8H20ClNO5 Molecular Weight (g/mol): 245.70 InChI Key: VEYRVLHAMHQVTC-UHFFFAOYSA-N IUPAC Name: 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol hydrochloride SMILES: Cl.OCCN(CCO)C(CO)(CO)CO

Pricing & Availability
Specifications

CAS	124763-51-5
Molecular Weight (g/mol)	245.70
SMILES	Cl.OCCN(CCO)C(CO)(CO)CO
IUPAC Name	2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol hydrochloride
InChI Key	VEYRVLHAMHQVTC-UHFFFAOYSA-N
Molecular Formula	C8H20ClNO5

327

TRIS, MP Biomedicals™

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.14 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Tris-[hydroxymethyl]aminomethane,THAM, 2-Amino-2-(hydroxymethyl)- 1,3-propandiol,Tromethamine,Trometamol

Pricing & Availability
Specifications

CAS	77-86-1
Molecular Weight (g/mol)	121.14
MDL Number	MFCD00004679
Synonym	Tris-[hydroxymethyl]aminomethane,THAM, 2-Amino-2-(hydroxymethyl)- 1,3-propandiol,Tromethamine,Trometamol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

328

Tricine, 0.5M buffer soln., pH 6.5, Thermo Scientific™

CAS: 4-1-5704 Molecular Formula: C6H13NO5 Molecular Weight (g/mol): 179.17 MDL Number: MFCD00004277 InChI Key: SEQKRHFRPICQDD-UHFFFAOYSA-N PubChem CID: 79784 ChEBI: CHEBI:39063 IUPAC Name: 2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acid SMILES: C(C(=O)O)NC(CO)(CO)CO

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Specifications

PubChem CID	79784
CAS	4-1-5704
Molecular Weight (g/mol)	179.17
ChEBI	CHEBI:39063
MDL Number	MFCD00004277
SMILES	C(C(=O)O)NC(CO)(CO)CO
IUPAC Name	2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acid
InChI Key	SEQKRHFRPICQDD-UHFFFAOYSA-N
Molecular Formula	C6H13NO5

329

Thermo Scientific Chemicals MES, 0.5M buffer soln., pH 5.0

CAS: 145224-94-8 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.25 MDL Number: MFCD00149409 InChI Key: MIIIXQJBDGSIKL-UHFFFAOYSA-N PubChem CID: 16218417 IUPAC Name: 2-(morpholin-4-yl)ethane-1-sulfonic acid hydrate SMILES: O.OS(=O)(=O)CCN1CCOCC1

Pricing & Availability
Specifications

PubChem CID	16218417
CAS	145224-94-8
Molecular Weight (g/mol)	213.25
MDL Number	MFCD00149409
SMILES	O.OS(=O)(=O)CCN1CCOCC1
IUPAC Name	2-(morpholin-4-yl)ethane-1-sulfonic acid hydrate
InChI Key	MIIIXQJBDGSIKL-UHFFFAOYSA-N
Molecular Formula	C6H15NO5S

330

Thermo Scientific Chemicals TRIS, 1.0M buffer soln., pH 7.2

Pricing & Availability
Specifications

PubChem CID	6503
CAS	77-86-1
Molecular Weight (g/mol)	121.136
ChEBI	CHEBI:9754
MDL Number	MFCD00004679
SMILES	C(C(CO)(CO)N)O
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

Life Science Buffers

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PIPES, Monosodium Salt, Biological Buffer, High Purity, 98.5%, Spectrum™ Chemical Small and Specialty Supplier PartnerSmall and/or specialty supplier based on Federal laws and SBA requirements.Learn More

Gibco™ PBS, pH 7.4 Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Bis-Tris Hydrochloride, Biological Buffer, 99%, Spectrum™ Chemical Small and Specialty Supplier PartnerSmall and/or specialty supplier based on Federal laws and SBA requirements.Learn More

PIPES, Monosodium Salt, Biological Buffer, High Purity, 98.5%, Spectrum™ Chemical

Gibco™ PBS, pH 7.4

Bis-Tris Hydrochloride, Biological Buffer, 99%, Spectrum™ Chemical