Life Science Buffers

Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.

Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.

Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.

The choice of buffer for a particular biological reaction or site depends on several factors:

Temperature
Desired or target pH
Buffer toxicity (to the system)
Buffer interactions with other system components

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316 – 330 of 3,503 results

316

Hanks' Balanced Salts, Without Sodium Bicarbonate, MP Biomedicals™

Hanks' Balanced Salts, Without Sodium Bicarbonate, Orange Powder

Pricing & Availability
Specifications

Color	Orange
Physical Form	Powder
pH	6.0 to 7.0 (before adjustment with sodium bicarbonate)

317

MP Biomedicals™ MOPS SDS Running Buffer for Bis-Tris Gels, Powder

Powder MOPS buffer salts to make PAGE buffer.

Pricing & Availability

318

Thermo Scientific Chemicals MOPS, 1.0M buffer soln., pH 8.0

CAS: 1132-61-2 Molecular Formula: C7H15NO4S Molecular Weight (g/mol): 209.26 MDL Number: MFCD00006183 InChI Key: DVLFYONBTKHTER-UHFFFAOYSA-N PubChem CID: 70807 ChEBI: CHEBI:44115 IUPAC Name: 3-morpholin-4-ylpropane-1-sulfonic acid SMILES: [O-]S(=O)(=O)CCC[NH+]1CCOCC1

Pricing & Availability
Specifications

PubChem CID	70807
CAS	1132-61-2
Molecular Weight (g/mol)	209.26
ChEBI	CHEBI:44115
MDL Number	MFCD00006183
SMILES	[O-]S(=O)(=O)CCC[NH+]1CCOCC1
IUPAC Name	3-morpholin-4-ylpropane-1-sulfonic acid
InChI Key	DVLFYONBTKHTER-UHFFFAOYSA-N
Molecular Formula	C7H15NO4S

319

MilliporeSigma™ HEPES, Free Acid, ULTROL™ Grade, Calbiochem™,

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.30 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N Synonym: N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic Acid PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid SMILES: OCCN1CCN(CCS(O)(=O)=O)CC1

Pricing & Availability
Specifications

PubChem CID	23831
CAS	7365-45-9
Molecular Weight (g/mol)	238.30
ChEBI	CHEBI:42334
MDL Number	MFCD00006158
SMILES	OCCN1CCN(CCS(O)(=O)=O)CC1
Synonym	N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic Acid
IUPAC Name	2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid
InChI Key	JKMHFZQWWAIEOD-UHFFFAOYSA-N
Molecular Formula	C8H18N2O4S

320

MilliporeSigma™ Imidazole, ULTROL™ Grade, Calbiochem™,

Useful in the pH range of 6.2 to 7.8

Pricing & Availability
Specifications

CAS	288-32-4
Molecular Weight (g/mol)	68.079
MDL Number	MFCD00005183
Grade	ULTROL
SMILES	N1C=CN=C1
Synonym	1,3-Diaza-2,4-cyclopentadiene
Recommended Storage	15°C to 30°C (Do not freeze); Keep container tightly closed. Keep container in a cool, well-ventilated area. Do not store above 20°C (68°F).
InChI Key	RAXXELZNTBOGNW-UHFFFAOYSA-N

321

HEPES Buffer, Powder, Cell Culture Grade, MP Biomedicals

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.30 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid SMILES: OCCN1CCN(CCS(O)(=O)=O)CC1

Pricing & Availability
Specifications

PubChem CID	23831
CAS	7365-45-9
Molecular Weight (g/mol)	238.30
ChEBI	CHEBI:42334
MDL Number	MFCD00006158
SMILES	OCCN1CCN(CCS(O)(=O)=O)CC1
IUPAC Name	2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid
InChI Key	JKMHFZQWWAIEOD-UHFFFAOYSA-N
Molecular Formula	C8H18N2O4S

322

MilliporeSigma™ HEPES, Free Acid, OmniPur™, Calbiochem™,

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.30 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N Synonym: N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic acid PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid SMILES: OCCN1CCN(CCS(O)(=O)=O)CC1

Pricing & Availability
Specifications

PubChem CID	23831
CAS	7365-45-9
Molecular Weight (g/mol)	238.30
ChEBI	CHEBI:42334
MDL Number	MFCD00006158
SMILES	OCCN1CCN(CCS(O)(=O)=O)CC1
Synonym	N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic acid
IUPAC Name	2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid
InChI Key	JKMHFZQWWAIEOD-UHFFFAOYSA-N
Molecular Formula	C8H18N2O4S

323

MP Biomedicals™ MES SDS Running Buffer for Bis-Tris Gels, Powder

Powder MES buffer salts to make PAGE buffer.

Pricing & Availability

324

Thermo Scientific Chemicals MES, 0.5M buffer soln., pH 5.5

CAS: 145224-94-8 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.25 MDL Number: MFCD00149409 InChI Key: MIIIXQJBDGSIKL-UHFFFAOYSA-N PubChem CID: 16218417 IUPAC Name: 2-(morpholin-4-yl)ethane-1-sulfonic acid hydrate SMILES: O.OS(=O)(=O)CCN1CCOCC1

Pricing & Availability
Specifications

PubChem CID	16218417
CAS	145224-94-8
Molecular Weight (g/mol)	213.25
MDL Number	MFCD00149409
SMILES	O.OS(=O)(=O)CCN1CCOCC1
IUPAC Name	2-(morpholin-4-yl)ethane-1-sulfonic acid hydrate
InChI Key	MIIIXQJBDGSIKL-UHFFFAOYSA-N
Molecular Formula	C6H15NO5S

325

TAPS sodium salt, 98%

CAS: 91000-53-2 Molecular Formula: C7H16NNaO6S Molecular Weight (g/mol): 265.256 MDL Number: MFCD00069903 InChI Key: FEGYIWVHCSRXCG-UHFFFAOYSA-M PubChem CID: 23667519 IUPAC Name: sodium;3-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]propane-1-sulfonate SMILES: C(CNC(CO)(CO)CO)CS(=O)(=O)[O-].[Na+]

Pricing & Availability
Specifications

PubChem CID	23667519
CAS	91000-53-2
Molecular Weight (g/mol)	265.256
MDL Number	MFCD00069903
SMILES	C(CNC(CO)(CO)CO)CS(=O)(=O)[O-].[Na+]
IUPAC Name	sodium;3-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]propane-1-sulfonate
InChI Key	FEGYIWVHCSRXCG-UHFFFAOYSA-M
Molecular Formula	C7H16NNaO6S

326

Thermo Scientific Chemicals BICINE, 0.5M buffer soln., pH 8.0

CAS: 150-25-4 Molecular Formula: C6H12NNaO4 Molecular Weight (g/mol): 185.16 MDL Number: MFCD00004295 InChI Key: MFBDBXAVPLFMNJ-UHFFFAOYSA-M PubChem CID: 8761 ChEBI: CHEBI:40957 IUPAC Name: 2-[bis(2-hydroxyethyl)amino]acetic acid SMILES: [Na+].OCCN(CCO)CC([O-])=O

Pricing & Availability
Specifications

PubChem CID	8761
CAS	150-25-4
Molecular Weight (g/mol)	185.16
ChEBI	CHEBI:40957
MDL Number	MFCD00004295
SMILES	[Na+].OCCN(CCO)CC([O-])=O
IUPAC Name	2-[bis(2-hydroxyethyl)amino]acetic acid
InChI Key	MFBDBXAVPLFMNJ-UHFFFAOYSA-M
Molecular Formula	C6H12NNaO4

327

Thermo Scientific Chemicals TRIS-Tricine-SDS running buffer (10X), cathode buffer, pH 8.3

Pricing & Availability
Specifications

Health Hazard 3	P264b-P280-P302+P352-P305+P351+P338-P332+P313-P362
Solubility Information	It is soluble in water.
Packaging	Plastic bottle
Physical Form	Liquid
Health Hazard 1	H315-H319-H335
Chemical Name or Material	TRIS-Tricine-SDS running buffer (10X)
TSCA	Yes
Recommended Storage	Ambient temperatures

328

MilliporeSigma™ TBS Tablets, Calbiochem™,

Dissolving one tablet in 500mL of deionized H₂O yields 150mM NaCl, 50mM Tris-HCl buffer

Pricing & Availability

329

Tricine, 0.5M buffer soln., pH 7.5, Thermo Scientific™

CAS: 4-1-5704 Molecular Formula: C6H13NO5 Molecular Weight (g/mol): 179.17 MDL Number: MFCD00004277 InChI Key: SEQKRHFRPICQDD-UHFFFAOYSA-N PubChem CID: 79784 ChEBI: CHEBI:39063 IUPAC Name: 2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acid SMILES: C(C(=O)O)NC(CO)(CO)CO

Pricing & Availability
Specifications

PubChem CID	79784
CAS	4-1-5704
Molecular Weight (g/mol)	179.17
ChEBI	CHEBI:39063
MDL Number	MFCD00004277
SMILES	C(C(=O)O)NC(CO)(CO)CO
IUPAC Name	2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acid
InChI Key	SEQKRHFRPICQDD-UHFFFAOYSA-N
Molecular Formula	C6H13NO5

330

Thermo Scientific Chemicals Phosphate-buffered saline (PBS, 10X), pH 7.6

Helps to maintain a constant pH

Pricing & Availability
Specifications

Physical Form	Liquid
pH	7.6
Chemical Name or Material	Phosphate Buffered Saline (PBS)
Recommended Storage	Ambient temperatures
Concentration	10 X

Life Science Buffers

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